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rabbit polyclonal anti ampkα1 2  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology rabbit polyclonal anti ampkα1 2
    Rabbit Polyclonal Anti Ampkα1 2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 257 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 257 article reviews
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    Effect of HS on hypothalamic expression of AMPK pathway in three modern broilers and their ancestor JF. Protein and RNA were extracted from hypothalamic tissues and analyzed by western blot (A,B) and qPCR (C–I) , respectively. Gene expression was determined by qPCR using 2 −ΔΔCT method and data are mean ± SEM ( n = 6/group). Protein expression was presented as ratio of p-AMPK <t>Thr172</t> /pan AMPK (A,B) . Protein was analyzed via AlphaVIew software and is expressed as mean ± SEM ( n = 3/group) with one representative blot shown (A) . Different letters indicate significant difference at P < 0.05. 95RAN, 1995 random bred; ACRB, Athens Canadian Random Bred; AMPK, AMP-activated protein kinase; E, environment; ExL, interaction between E and L; HS, heat stress (36°C); JF, jungle fowl; L, line; MRB, modern random bred; TN, thermoneutral (25°C).
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    Effect of HS on hypothalamic expression of AMPK pathway in three modern broilers and their ancestor JF. Protein and RNA were extracted from hypothalamic tissues and analyzed by western blot (A,B) and qPCR (C–I) , respectively. Gene expression was determined by qPCR using 2 −ΔΔCT method and data are mean ± SEM ( n = 6/group). Protein expression was presented as ratio of p-AMPK <t>Thr172</t> /pan AMPK (A,B) . Protein was analyzed via AlphaVIew software and is expressed as mean ± SEM ( n = 3/group) with one representative blot shown (A) . Different letters indicate significant difference at P < 0.05. 95RAN, 1995 random bred; ACRB, Athens Canadian Random Bred; AMPK, AMP-activated protein kinase; E, environment; ExL, interaction between E and L; HS, heat stress (36°C); JF, jungle fowl; L, line; MRB, modern random bred; TN, thermoneutral (25°C).
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    Effect of HS on hypothalamic expression of AMPK pathway in three modern broilers and their ancestor JF. Protein and RNA were extracted from hypothalamic tissues and analyzed by western blot (A,B) and qPCR (C–I) , respectively. Gene expression was determined by qPCR using 2 −ΔΔCT method and data are mean ± SEM ( n = 6/group). Protein expression was presented as ratio of p-AMPK <t>Thr172</t> /pan AMPK (A,B) . Protein was analyzed via AlphaVIew software and is expressed as mean ± SEM ( n = 3/group) with one representative blot shown (A) . Different letters indicate significant difference at P < 0.05. 95RAN, 1995 random bred; ACRB, Athens Canadian Random Bred; AMPK, AMP-activated protein kinase; E, environment; ExL, interaction between E and L; HS, heat stress (36°C); JF, jungle fowl; L, line; MRB, modern random bred; TN, thermoneutral (25°C).
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    Effect of HS on hypothalamic expression of AMPK pathway in three modern broilers and their ancestor JF. Protein and RNA were extracted from hypothalamic tissues and analyzed by western blot (A,B) and qPCR (C–I) , respectively. Gene expression was determined by qPCR using 2 −ΔΔCT method and data are mean ± SEM ( n = 6/group). Protein expression was presented as ratio of p-AMPK Thr172 /pan AMPK (A,B) . Protein was analyzed via AlphaVIew software and is expressed as mean ± SEM ( n = 3/group) with one representative blot shown (A) . Different letters indicate significant difference at P < 0.05. 95RAN, 1995 random bred; ACRB, Athens Canadian Random Bred; AMPK, AMP-activated protein kinase; E, environment; ExL, interaction between E and L; HS, heat stress (36°C); JF, jungle fowl; L, line; MRB, modern random bred; TN, thermoneutral (25°C).

    Journal: Frontiers in Veterinary Science

    Article Title: Effect of Cyclic Heat Stress on Hypothalamic Oxygen Homeostasis and Inflammatory State in the Jungle Fowl and Three Broiler-Based Research Lines

    doi: 10.3389/fvets.2022.905225

    Figure Lengend Snippet: Effect of HS on hypothalamic expression of AMPK pathway in three modern broilers and their ancestor JF. Protein and RNA were extracted from hypothalamic tissues and analyzed by western blot (A,B) and qPCR (C–I) , respectively. Gene expression was determined by qPCR using 2 −ΔΔCT method and data are mean ± SEM ( n = 6/group). Protein expression was presented as ratio of p-AMPK Thr172 /pan AMPK (A,B) . Protein was analyzed via AlphaVIew software and is expressed as mean ± SEM ( n = 3/group) with one representative blot shown (A) . Different letters indicate significant difference at P < 0.05. 95RAN, 1995 random bred; ACRB, Athens Canadian Random Bred; AMPK, AMP-activated protein kinase; E, environment; ExL, interaction between E and L; HS, heat stress (36°C); JF, jungle fowl; L, line; MRB, modern random bred; TN, thermoneutral (25°C).

    Article Snippet: The primary antibodies used were rabbit polyclonal anti-phospho AMPKα1/2 Thr172 (#2531, Cell Signaling Technology, Danvers, MA), anti-AMPKα1/2 (#2795, Cell Signaling Technology, Danvers, MA), anti-HSP90 (#PA5-17610, Pierce Thermo Scientific, Rockford, IL), goat polyclonal anti-HSP60 (#sc-1052, Santa Cruz Biotechnology, Dallas, TX), mouse monoclonal anti-HSP70 (#MAI-91159, Pierce Thermo Scientific, Rockford, IL) and rabbit anti-GAPDH (Santa Cruz Biotechnology, Dallas, TX).

    Techniques: Expressing, Western Blot, Gene Expression, Software